Description
Section 1: Biochemistry and molecular biology of viral proteins 1. The HIV life cycle 2. Reverse transcriptase 3. Ribonuclease H 4. Integrase 5. Protease 6. Expression of gag, envelope and other HIV antigens using Bacculovirus 7. Envelope expression and purification 8. HIV envelope expression and purification 9. RNA binding assays for the regulatory proteins tat and rev 10. Cellular and cell-free assays for tat 11. Cellular assays for rev 12. DNA binding proteins interacting with the viral LTR 13. Nef 14. Vpu 15. Vif Section 2: Drug discovery 16. Cellular assays for antiviral drugs 17. Inhibition of HIV by ribozymes and antisense oligonucleotides 18. Gene therapy approaches to the inhibition of HIV
